dot blot based proteome profiler human xl cytokine antibody array kit (R&D Systems)
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dot blot based proteome profiler human xl cytokine antibody array kit
Dot Blot Based Proteome Profiler Human Xl Cytokine Antibody Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dot+blotting+kit/Proteome+Profiler+Human+XL+Cytokine+Array+Kit/pm40492327-92-10-20
Average 96 stars, based on 513 article reviews
Dot Blot Based Proteome Profiler Human Xl Cytokine Antibody Array Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 513 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dot+blotting+kit/Proteome+Profiler+Human+XL+Cytokine+Array+Kit/pm40492327-92-10-20
Average 96 stars, based on 513 article reviews
dot blot based proteome profiler human xl cytokine antibody array kit - by Bioz Stars,
2026-09
96/100 stars
Images
1) Product Images from "Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models."
Article Title: Fibroblast proximity to a tumor impacts fibroblast extracellular vesicles produced by 3D bioprinted stromal models.
Journal: Biomaterials science
doi: 10.1039/d4bm01569j
Figure Legend Snippet: Fig. 4 NTAF media has increase in cancer progression related cytokines. (A) (i) Top four cytokines expressed in the cytokine array. (ii) Middle two cytokines expressed across the four model groups. (iii) Lower middle expressed cytokines in the cytokine array. (iv) Lowest expressed cytokines.
Techniques Used:
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Cell Culture:Article Title: Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation Article Snippet: HCAECs and HITAECs cultured in 6-well plates (85–90% confluence) were exposed to 100 μL of MPPs, CPP-P, CPP-S (OD 650 = 0.08–0.10, for CPP-P and CPP-S: equal to 50 μg calcium or 1.2 × 10 5 OsteoSense 680EX-positive PKH67-negative events), or PBS for 4 h. Upon the collection of the conditioned medium (300 μL per well), the levels of interleukin-6 and -8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay using the respective kits (ab178013 and ab46032, Abcam, Cambridge, UK) according to the manufacturer’s protocol. .. Alternatively, HCAECs and HITAECs cultured in T-75 flasks (85–90% confluence) were exposed to 1000 μL of CPP-P (OD 650 = 0.08–0.10, equal to 500 μg calcium or 1.2 × 10 6 OsteoSense 680EX-positive PKH67-negative events) or PBS for 4 h. Upon the collection of the conditioned medium (2 mL per flask) and cell lysis by RIPA buffer (89901, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with protease and phosphatase inhibitors (78444, Thermo Fisher Scientific, Waltham, MA, USA), we profiled the conditioned medium and cell lysate (1 mg protein) for a wide spectrum of cytokines using the respective Lysis:Article Title: Calciprotein Particles Link Disturbed Mineral Homeostasis with Cardiovascular Disease by Causing Endothelial Dysfunction and Vascular Inflammation Article Snippet: HCAECs and HITAECs cultured in 6-well plates (85–90% confluence) were exposed to 100 μL of MPPs, CPP-P, CPP-S (OD 650 = 0.08–0.10, for CPP-P and CPP-S: equal to 50 μg calcium or 1.2 × 10 5 OsteoSense 680EX-positive PKH67-negative events), or PBS for 4 h. Upon the collection of the conditioned medium (300 μL per well), the levels of interleukin-6 and -8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay using the respective kits (ab178013 and ab46032, Abcam, Cambridge, UK) according to the manufacturer’s protocol. .. Alternatively, HCAECs and HITAECs cultured in T-75 flasks (85–90% confluence) were exposed to 1000 μL of CPP-P (OD 650 = 0.08–0.10, equal to 500 μg calcium or 1.2 × 10 6 OsteoSense 680EX-positive PKH67-negative events) or PBS for 4 h. Upon the collection of the conditioned medium (2 mL per flask) and cell lysis by RIPA buffer (89901, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with protease and phosphatase inhibitors (78444, Thermo Fisher Scientific, Waltham, MA, USA), we profiled the conditioned medium and cell lysate (1 mg protein) for a wide spectrum of cytokines using the respective |